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Analysis of ergot alkaloids from transformed strains. HPLC chromatograms of recipient strain (N. fumigata easM knockout), transformed strains containing E. typhina × E. festucae isolate Lp1 cloA or synthetic C. africana cloA, and DHLA standard. Fluorescence was detected at 372 nm after exciting at 272 nm. Mass spectra of fragmented parent ion of m/z 271.2 from DHLA standard and N. fumigata expressing synthetic C. africana cloA are inserted in relevant chromatograms.
