TABLE 1.

Oxidation of exogenously fed agroclavine to lysergic acid by N. fumigata easM knockout cultures expressing different alleles of cloA

Straina	Treatmentb	Agroclavine (nmol/culture [mean ± SE])	Lysergic acid (nmol/culture [mean ± SE])
easM ko	Agroclavine	12.3 ± 2.3 Ac	NDd
easM ko + Epichloë sp. Lp1 cloA	Agroclavine	11.0 ± 1.3 A	0.25 ± 0.02 A
easM ko + C. africana synthetic cloA	Agroclavine	12.4 ± 0.7 A	0.47 ± 0.02 B
Medium	Agroclavine	11.3 ± 2.7 A	ND
easM ko + Epichloë sp. Lp1 cloA	Methanol	ND	ND
easM ko + C. africana cloA	Methanol	ND	ND

^an = 6.

^bAgroclavine, 20 nmol.

^cMeans followed by the letter A did not differ significantly in an ANOVA (P > 0.05).

^dND, not detected; limit of detection = 0.01 pmol/culture.