TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK− mK−) phoA λ− | Novagen |
| WM3064 | thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 dapA1341::[erm pir] | W. Metcalf |
| A. tertiaricarbonis strains | ||
| RN12 | Floc-forming strain | This study |
| RN12T4 mutant | mariner transposon insertion in rpoN1 at nt 1274 | This study |
| RN12T13 mutant | mariner transposon insertion in gt1 at nt 710 | This study |
| RN12P20 mutant | mariner transposon insertion in uge at nt105 | This study |
| Plasmids | ||
| pFAC | mariner transposon delivery plasmid R6K, Gmr | 13 |
| pminiHmarRB1 | mariner transposon delivery plasmid R6K, Kmr | 58 |
| pBBR1MCS-2 | Broad-host-range shuttle vector | 59 |
| pBBR1MCS2-rpoN1 | rpoN1 gene cloned in pBBR1MCS-2, Kmr | This study |
| pBBR1MCS2-rpoN2 | rpoN2 gene cloned in pBBR1MCS-2, Kmr | This study |
| pBBR1MCS2-rpoN3 | rpoN3 gene cloned in pBBR1MCS-2, Kmr | This study |
| pBBR1MCS2-rpoN4 | rpoN4 gene cloned in pBBR1MCS-2, Kmr | This study |
| pBBR1MCS2-gt1 | gt1 gene cloned in pBBR1MCS-2, Kmr | This study |
| pBBR1MCS2-uge | uge gene cloned in pBBR1MCS-2, Kmr | This study |
a
Gmr, gentamicin resistance; Kmr, kanamycin resistance.