TABLE 3.

Primers used in this study

Primer use and name	Sequence (5′–3′)	Restriction site
Complementation primers
rpoN1F	5′-CGGAATTCATGAAACCCTCGCTGCAAATCCG-3′	EcoRI at 5′ end
rpoN1R	5′-GCTCTAGAGCAGGAACAGGGCAATCTGGGGC-3′	XbaI at 5′ end
rpoN2F	5′-GGAATTCACACTGTTTCTAACGCCTACC-3′	EcoRI at 5′ end
rpoN2R	5′-GCTCTAGACGACGAGTTCTAGGGTTTCAG-3′	XbaI at 5′ end
rpoN3F	5′-GGAATTCCGGAGTACCTTGTCCCATG-3′	EcoRI at 5′ end
rpoN3R	5′-GCTCTAGATTCAACCACCACAAGGAGC-3′	XbaI at 5′ end
rpoN4F	5′-GGAATTCGCGCACATCACACCTGAAGAG-3′	EcoRI at 5′ end
rpoN4R	5′-GCTCTAGAATAAGGGCCTGACTAAGCGAC	XbaI at 5′ end
gt1-F	5′-GGAATTCAACCCCAGCAACGCCAAGTA-3′	EcoRI at 5′ end
gt1-R	5′-GCTCTAGAAGGAAGGCATGGGTGAAGG-3′	XbaI at 5′ end
uge-F	5′-CGGAATTCTGCCCACTCCATCATGAAAAT-3′	EcoRI at 5′ end
uge-R	5′-GCTCTAGACGATCAGACCTTGTAGTAGCC-3′	XbaI at 5′ end
Real-time PCR primers
Q-gt1-F	5′-GCGGTGATCGTGTTCTCGTC-3′
Q-gt1-R	5′-GGCGTATTCGGTCCACTTGG-3′
Q-uge-F	5′-CCGACAACCTCAACGCCTACT-3′
Q-uge-R	5′-CATCTGCTGGAAGCGGAAAC-3′
Q-Tap F	5′-AGTTCACGGAGGTCGTTTCG-3′
Q-Tap R	5′-AGCAATCACGCCTTGGGTAG-3′
Q-16S-F	5′-CGTGTAGCAGTGAAATGCGTAG-3′
Q-16S-R	5′-GAAACCCTCCCAACAACCAG-3′
RT-PCR primers
RT-TapA-F	5′-CTACACCAAGAAGGCGAAGT-3′
RT-TapA-R	5′-AGGAGCAGGTGCCCGATT-3′
RT-16S-F	5′-CTGAGAGGACGACCAGCCACAC-3′
RT-16S-R	5′ -TACGCATTTCACTGCTACACGC-3′
Primer extension primers
tapA-SP1	5′-GCCGTCGGAGTAATAATGTA-3′
tapA-SP2	5′-CGTTAGCGATTGCGATAGA-3′
uge-SP1	5′-CTCGGAAAACGGCATCT-3′
uge-SP2	5′-GAGCTGGAGGCATACAC-3′
oligo(dT)	5′-GCCAGTCTTTTTTTTTTTTTTTTT-3′