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Activity assay of purified rubber oxygenases. Purified preparations of RoxBXsp, LcpK30, or RoxAXsp (4 μg of each protein) were added to polyisoprene latex (arrow) in an OXY-4 miniapparatus at 30°C. The concentration of dissolved oxygen was monitored over time (A). The blank corresponds to assay buffer without enzyme. The produced products were solvent extracted and analyzed by HPLC (B). Note formation of identical products for LcpK30 and RoxBXsp, while RoxAXsp produced mainly ODTD.
