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pH profile and inhibition assay of RoxBXsp. The activity of purified RoxBXsp was assayed at different pH values, as indicated (A). The activities of purified LcpK30 (top bar) and of RoxBXsp in the absence or presence of potential inhibitors are shown in panel B. The values show the relative peak areas of the 23.7-min product peak (C35 oligo-isoprenoid). Each experiment was performed in duplicate.
