TABLE 2.
Exclusivity of PCR primers and probes was confirmed by testing 105 copies of purified DNA from each of the 22 non-Salmonella target organisms in the multiplex real-time PCR
| Control organism | Result by PCR targeta |
|||
|---|---|---|---|---|
| invA | prt | Sdf-1 | IAC | |
| Escherichia coli (ATCC 8759) | Neg | Neg | Neg | Pos |
| E. coli (ATCC 43888) | Neg | Neg | Neg | Pos |
| E. coli (ATCC 43895) | Neg | Neg | Neg | Pos |
| E. coli (ATCC 6209) | Neg | Neg | Neg | Pos |
| E. coli (ATCC 6210) | Neg | Neg | Neg | Pos |
| Shigella flexneri (ATCC 29903) | Neg | Neg | Neg | Pos |
| Cronobacter sakazakii (ATCC 12868) | Neg | Neg | Neg | Pos |
| Enterobacter aerogenes (ATCC 13048) | Neg | Neg | Neg | Pos |
| Enterobacter cloacae (lab isolate) | Neg | Neg | Neg | Pos |
| Citrobacter freundii (lab isolate) | Neg | Neg | Neg | Pos |
| Klebsiella pneumoniae (ATCC 13883) | Neg | Neg | Neg | Pos |
| Proteus hauseri (ATCC 13315) | Neg | Neg | Neg | Pos |
| Pseudomonas aeruginosa (ATCC 9027) | Neg | Neg | Neg | Pos |
| Pantoea sp. (lab isolate) | Neg | Neg | Neg | Pos |
| Vibrio cholerae (ATCC 35971) | Neg | Neg | Neg | Pos |
| Yersinia enterocolitica (ATCC 23715) | Neg | Neg | Neg | Pos |
| Acinetobacter sp. (lab isolate) | Neg | Neg | Neg | Pos |
| Bacillus subtilis (ATCC 6633) | Neg | Neg | Neg | Pos |
| Staphylococcus aureus (ATCC 6538) | Neg | Neg | Neg | Pos |
| Rhodococcus equi (ATCC 6939) | Neg | Neg | Neg | Pos |
| Listeria monocytogenes (ATCC 19115) | Neg | Neg | Neg | Pos |
| Listeria innocua (ATCC 33090) | Neg | Neg | Neg | Pos |
| No-template control | Neg | Neg | Neg | Pos |
| S. Enteritidis (ATCC 13076) | Pos | Pos | Pos | Pos |
a
The results shown here were obtained after 40 cycles of PCR. Pos, positive (displayed a CT value in the PCR); Neg, negative (displayed no CT value in the PCR).