TABLE 4.
Comparison of results obtained from the PCR, VIDAS, and culture methodsa
| Source (no. of samples) | PCR result or CT value | VIDAS result | Culture resultb |
|---|---|---|---|
| Cheese product plant (132) | 22.49 | Negative | Positive |
| 38.13 | Negative | Positive | |
| 37.79 | Negative | Negative | |
| Cheese product company (140) | 25.75 | Negative | Positive |
| 26.31 | Positive | Positive | |
| 27.25 | Positive | Positive | |
| 28.84 | Positive | Positive | |
| 31.26 | Positive | Positive | |
| 34.08 | Negative | Positive | |
| 34.40 | Negative | Positive | |
| Negative | Positive | Negative | |
| Whey powder company (154) | All negative | 7 positives | 7 of 7 negative |
| 6 food product companies (686) | 36.64 | All negative | 1 of 1 negative |
| 3 dairy product companies (309) | All negative | All negative | Not performed |
| 2 food and cheese companies (320) | All negative | All negative | Not performed |
| Total (1,741) | 11 positive | 12 positive | 9 positive |
A total of 1,741 environmental samples from 14 food-processing facilities were screened by PCR and VIDAS methods. Culture analyses were performed for a total of 19 samples that were positive according to the PCR or VIDAS method. Salmonella isolates were obtained from 9 of 11 PCR-positive samples and 4 of 12 VIDAS-positive samples. VIDAS failed to detect Salmonella in 5 culture-positive samples.
Culture analyses were performed for all samples that were positive with PCR or VIDAS screening.