Validation of the A. actinomycetemcomitans ordered library. P, plate; W, well; Site, transposon insertion site in the VT1169 genome; Product, gene product nearest the transposon insertion; PTS, phosphotransferase; Ltx, leukotoxin. Each box under the Sanger and PCR headings indicates a replicate. Green, positive Sanger/PCR result; red, negative Sanger/PCR result.