FIG 6.

Differences in genome arrangement between previously published genome sequence (WT-Ref) and resequencing results (WT-Parent). (A) The overall arrangement of the wild-type genome sequenced by the two methods. There is only one major disagreement at the glucan degradation locus (GDL). The black lines at each region show the locations of the PCR products used to validate the biologically relevant genome region. (B) PCR validation of the arrangement at the GDL. Expected product sizes for the resequenced genome are AC, 10.0 kb; CB, 12.0 kb; and BD, 7.9 kb. Product sizes for the published genome are AB, 10.5 kb; BC, 9.0 kb; and CD, 10.6 kb. The expected products were attained for the orientation reported by the PacBio resequencing. For reactions targeting the orientation in the published genome, one larger than expected product was observed for reaction AB, and all other bands were faint or smaller than expected, indicative of nonspecific PCR products.