. 2017 Jun 30;83(14):e00444-17. doi: 10.1128/AEM.00444-17

TABLE 3.

Primers used in this study, including those used to amplify IS element probes for Southern blot analysis, GDL arrangement confirmation, and ISCbe3 duplication confirmation

Target	Primer name	Sequence (5′ to 3′)
ISCbe4	JD001	GGAGCGATAATAGAGAGAGTAGTAGAC
ISCbe4	JD002	CTTTATCCAATTAGCTCCATCTCC
ISCbe1	JD003	GCATACTTCAACATCCCTATCACAG
ISCbe1	JD004	GTACGTATATGCGACGATACAAGC
ISCbe2	JD005	CTGGCTTCAGATACAGACG
ISCbe2	JD006	GCTCTTGGTGGAATAGGATTTAGTTC
ISCbe3	JD007	CAGTCAAGGGTGTTTATGAG
ISCbe3	JD008	CACCCTGATATGGCAGTATC
ISCbe6	JD009	CTCAACTGGTGGATTATACATC
ISCbe6	JD010	CCAAGAGACAGAGAAGGTG
GDL A	AR073	CCACTTGGTGCCACATAAATAGC
GDL B	AR075	GCAAGAAGGTTAGGTGGAAACAG
GDL B	AR076	CTGTTTCCACCTAACCTTCTTGC
GDL C	AR077	AAGAAGAAATTCAATCAAAGTTGATG
GDL C	AR074	TCACGTATGACGATTGAAGC
GDL D	AR078	GAGGTTAGAGATTTATGAAGCGTTACAG
ISCbe3 region A	AR082	TAAAAGCTGTATCGCACCACC
ISCbe3 region A	AR084	AATTGAAGCAGAGTGTGGAGC
ISCbe3 region B	AR083	CTCAGCTTATTCAAGGACGAC
ISCbe3 region B	AR081	CACTTCTCAGTGGAGTAGAGTC