FIG 1.

Flow chart of the procedure to enrich Nitrotoga-like bacteria from sand in an eelgrass zone. All cultures for enrichment were incubated in mineral medium containing 0.5 to 1.4 mM NaNO₂ in the dark without shaking. The cultures were transferred with inocula of 1 or 10% (vol/vol), except when serial dilutions were used. In growth tests, + indicates the relative cell growth rate in batch cultures.