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Representative data of half-saturation constants (apparent Km) for nitrite of exponential-growth-phase cells of AM1 at 16°C without shaking. (A) The initial nitrite concentration was adjusted to 0.3 mM. The samples were taken out every 2.5 to 20 min. (B) Michaelis-Menten plots were fitted by the least-squares method. The Km value and maximum cell activity were calculated from biological 3-fold measurements.
