. 2017 Jun 30;83(14):e00549-17. doi: 10.1128/AEM.00549-17

TABLE 2.

Comparison of characteristics among Nitrotoga-enriched cultures investigated in detail

Characteristic	AM1	“Ca. Nitrotoga arctica”^a	HW29^b
Sample source	Coastal sand	Permafrost-affected soil	RAS (fish tank)
Tendency to aggregate	Weak	Weak	Weak
Overall appearance	Wrinkled rod shape	Irregular coccoid	Irregular coccoid
Optimum temp (°C)	16	13	22
Applied pH for cultivation	8.0–8.3 (7.8)^c	7.4–7.6	6.8–7.4
K_m (μM)	24.7 ± 9.8	58 ± 28	ND
Maximum cell activity (fmol · cell⁻¹ · h⁻¹)	6.1 ± 0.2	2	ND
Generation time (h)	54	44	ND

Information is from references 23 and 24.

Information is from reference 23. RAS, recirculation aquaculture system; ND, no data.

pH values applied for initial incubation with 2% NaCl, which was not an effective enrichment procedure.