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Strain YIGS16 has more ERESs and a reduced amount of ER membrane compared to strain AACK. (a) Airyscan images showing ERESs by the colocalization of Sec16-GFP and Sec13-RFP. SEC16 was fused with GFP, and SEC13 was fused with RFP in strains AACK and YIGS16. (b) ER staining was performed, and the ER membrane changes were quantified by their fluorescence intensity. *, P < 0.05; **, P < 0.01. The data are reported as the mean values ± standard deviations from independent triplicates.
