TABLE 2.
Plasmids and strains used in this study
| Name | Description | Origin or reference |
|---|---|---|
| Plasmids | ||
| CPOTud | TPI promoter and terminator from S. cerevisiae, POT marker from Schizosaccharomyces pombe (2μ) | 7 |
| pAlphaAmyCPOT | α-Factor leader with α-amylase gene inserted into CPOTud | 7 |
| p416TEF | TEF1 promoter (centromeric) | 22 |
| p426TEF | TEF1 promoter (2μ) | 22 |
| p416TEF-SAR1 | PTEF1-SAR1 | This study |
| p416TEF-SEC16 | PTEF1-SEC16 | This study |
| p416TEF-SEC2 | PTEF1-SEC2 | This study |
| p416TEF-SEC4 | PTEF1-SEC4 | This study |
| p416TEF-SEC15 | PTEF1-SEC15 | This study |
| p416TEF-YPT32 | PTEF1-YPT32 | This study |
| p426TEF-SEC16 | PTEF1-SEC16 (2μ) | This study |
| pAlphaTrEGCPOT | α-Factor leader with endoglucanase I gene inserted into CPOTud | This study |
| pCP-aGLA | α-Factor leader with glucan 1,4-α-glucosidase gene inserted into CPOTud | 3 |
| Strains | ||
| CEN.PK530-1C | MATa URA3 HIS3 LEU2 TRP1 SUC2 MAL2-8c tpi1(41-707)::loxP-KanMX4-loxP | 7 |
| CEN.PK530-1CK | MATa URA3 HIS3 LEU2 TRP1 SUC2 MAL2-8c tpi1(41-707)::loxP | This study |
| CEN.PK530-1D | MATa ura3-52 HIS3 LEU2 TRP1 SUC2 MAL2-8c tpi1(41-707)::loxP-KanMX4-loxP | 6 |
| CEN.PK530-6CK | MATa URA3 HIS3 LEU2 TRP1 SUC2 MAL2-8c tpi1(41-707)::loxP PGPD-SEC16 | This study |
| YPAE | CEN.PK530-1D with pAlphaAmyCPOT and p416TEF | This study |
| YPASAR1 | CEN.PK530-1D with pAlphaAmyCPOT and p416TEF-SAR1 | This study |
| YPASEC16 | CEN.PK530-1D with pAlphaAmyCPOT and p416TEF-SEC16 | This study |
| YPASEC2 | CEN.PK530-1D with pAlphaAmyCPOT and p416TEF-SEC2 | This study |
| YPASEC4 | CEN.PK530-1D with pAlphaAmyCPOT and p416TEF-SEC4 | This study |
| YPAYPT32 | CEN.PK530-1D with pAlphaAmyCPOT and p416TEF-YPT32 | This study |
| YPA2uE | CEN.PK530-1D with pAlphaAmyCPOT and p426TEF | This study |
| YPA2uSEC16 | CEN.PK530-1D with pAlphaAmyCPOT and p426TEF-SEC16 | This study |
| AACK | CEN.PK530-1CK with pAlphaAmyCPOT | This study |
| YIGS16 | CEN.PK530-6CK with pAlphaAmyCPOT | This study |
| AACK-16G13R | AACK SEC16-GFP; SEC13-RFP | This study |
| YIGS16-16G13R | YIGS16 SEC16-GFP; SEC13-RFP | This study |
| Y1EK | CEN.PK530-1CK with CPOTud | This study |
| Y6EK | CEN.PK530-6CK with CPOTud | This study |