TABLE 3.
Primers used in this study
| Name | Sequence (5′ to 3′) |
|---|---|
| sar1F | GCAATCTAATCTAAGTTTTCTAGAACTAGTGGATCCCCCGGGAACAAAATGGCTGGTTGGGATATTTTTGGTTGGTTCAGAGATGTGTTGGCTTCCCTTGGTCTGTGGA |
| sar1R | CATAACTAATTACATGACTCGAGGTCGACGGTATCGATAAGCTTGTTAAATATATTGAGATAACCATTGGAACGCCT |
| p416TEF-PTEF-F | CCGGGGGATCCACTAGTTC |
| p416TEF-TCYC1-R | CAAGCTTATCGATACCGTCG |
| sec2F | GCATAGCAATCTAATCTAAGTTTTCTAGAACTAGTGGATCCAACAAAATGGATGCTTCTGAGGAAGCAAA |
| sec2R | CATAACTAATTACATGACTCGAGGTCGACGGTATCGATAAGCTTGTTATTGCTGTTCCTGGGCATC |
| sec4F | GCATAGCAATCTAATCTAAGTTTTCTAGAACTAGTGGATCCAACAAAATGTCAGGCTTGAGAACTGTTTC |
| sec4R | CGTGACATAACTAATTACATGACTCGAGGTCGACGGTATCGATTCAACAGCAATTTGATTTAGAAC |
| sec15F | GCATAGCAATCTAATCTAAGTTTTCTAGAACTAGTGGATCCAACAAAATGGACCAAGAAGGCCAGCCAGTT |
| sec15R | GCGTGACATAACTAATTACATGACTCGAGGTCGACGGTATCGATTTAACGTCTATTAAAAAATTTGGC |
| sec16F | GCATAGCAATCTAATCTAAGTTTTCTAGAACTAGTGGATCCAACAAAATGACACCTGAAGCCAAGAA |
| sec16R | CGTGACATAACTAATTACATGACTCGAGGTCGACGGTATCGATTTATTGTATGTTATCCATTAC |
| ypt32F | GCATAGCAATCTAATCTAAGTTTTCTAGAACTAGTGGATCCAACAAAATGAGCAACGAAGATTACGG |
| ypt32R | GCGTGACATAACTAATTACATGACTCGAGGTCGACGGTATCGATTTAACAACAGTTGCTGGAT |
| Psec16-GPD-R | ATTATTTACGTATTCTTTGAAATGGCGAGTATTGATAATGATAAACTGAGAGAAGGAGAAAGCACTTAAAAG |
| Psec16-GPD-F | CTTCTTTTAAGTGCTTTCTCCTTCTCTCAGTTTATCATTATCAATACTCGCCATTTC |
| SEC16pro-L-F | GTCAGGGTGTTCTTCGATTTTCC |
| SEC16pro-L-R | GTCAAGGAGGGTATTCTGGGCCTCCATGTCATGGCTCGTCATGGAAAAGGCC |
| amdSYM-F | GACATGGAGGCCCAGAATAC |
| amdSYM-R | CAGTATAGCG ACCAGCATTC |
| SEC16pro-rec-F | GTATGTGAATGCTGGTCGCTATACTGTCGGTTAACCTTGGTTGTTGC |
| SEC16pro-rec-R | ATGGCTCGTCATGGAAAAGGCC |
| GPDpro-F | GTTAGTTTTTGGGCCTTTTCCATGACGAGCCATAGTTTATCATTATCAATACTCGCCATTTCAAAGAATACG |
| GPDpro-R | GGTTTTTCCTTTTCTTGGCTTCAGGTGTCATTTTGTTATCCGTCGAAACTAAGTTCTGGTGTTTTAAAACTAAAAAAAAGAC |
| SEC16pro-R-F | ATGACACCTGAAGCCAAGAAAAGGAAAAACCAAAAGAAG |
| SEC16pro-R-R | CTCTTCGTATTCTTGAGACATGGC |
| sec16-end-w/o TAA-F | GGTTGGTTGAAGAAAGATACTGGC |
| sec16-end-w/o TAA-R | TTGTATGTTATCCATTACATTAACATAGCC |
| sec16-G4S-GFP-F | GAAGAAAACAGCGAGAGGCTATGTTAATGTAATGGATAACATACAAGGTGGAGGTGGATCTATGCGAATCCCCGGGTTAATTAAC |
| GFP-R | CTATTTGTATAGTTCATCCATGCCATGTG |
| GFP-tSEC16-F | GCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAGTTATAGTAATACGTTTTTCTTTCATGGTACCC |
| tSEC16-R | GGTATGATAGAATAATATTAGTATAAGCAAAGTTTCAG |
| tSEC16-amds-f | GATGAATGCGATCTGAAACTTTGCTTATACTAATATTATTCTATCATACCGACATGGAGGCCCAGAATACCCTCC |
| amds-R | CAGTATAGCGACCAGCATTCACATACG |
| amds-tSEC16-overlap-f | GAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTGTTATAGTAATACGTTTTTCTTTCATGGTACCC |
| tSEC16-overlap-R | AGTACAAGACTTTTCAAATGCTAACG |
| sec13-end-w/o TAA-F | CCATTGGCGTTAACTCTGCTTC |
| sec13-end-w/o TAA-R | CTGATGAACTTCACCAGCGGGTTC |
| sec13-G4S-RFP-F | GGAAAATCTTGAGGGTAAATGGGAACCCGCTGGTGAAGTTCATCAGGGAGGAGGTGGTTCTATGGCCTCCTCCGAGGACGTCATC |
| RFP-R | TTAGGCGCCGGTGGAGTGGCGGCCC |
| RFP-tSEC13-F | GTGGAACAGTACGAGCGCGCCGAGGGCCGCCACTCCACCGGCGCCTAAAGAGTATCAAGAATTTAAAATGAAACATCTCAAAAGAAAAAAG |
| tSEC13-R | GTTCGCTTGTTTTTGTATAAGAAATATCTATGAC |
| tSEC13-amds-f | CTTCTTATATTAATATTGTCATAGATATTTCTTATACAAAAACAAGCGAACGACATGGAGGCCCAGAATACCCTCC |
| amds-R | CAGTATAGCGACCAGCATTCACATACG |
| amds-tSEC13-overlap-f | GAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTGAGAGTATCAAGAATTTAAAATGAAACATCTCAAAAGAAAAAAG |
| tSEC13-overlap-R | GGATGTTGGGAGGAGATGCTGTCG |
| KpnI-alpha-leader-F | AAGGTACCAACAAAATGAGATTTCCATCTATTTTTACTGC |
| XhoI-cellulase-R | GAAAAGAAGATAATATTTTTATATAATTATATTAATCTTAGTTTCTAG |
| qpcr-ACT1-F | GCCTTCTACGTTTCCATCCA |
| qpcr-ACT1-R | GGCCAAATCGATTCTCAAAA |
| qpcr-sar1-f | AGCTCGTCGTTTATGGAAGGA |
| qpcr-sar1-r | GCGTTTGGAGCATCGATCTT |
| qpcr-sec2-f | GTGTTTGCTAAAGTTGCGGC |
| qpcr-sec2-r | ACCCTGCTGTCTCTTTTGGT |
| qpcr-sec4-f | TGGTGATTCTGGTGTTGGGA |
| qpcr-sec4-r | CCAGCGGTATCCCAAAGTTG |
| qpcr-sec15-f | TGCATGACACACCAAGTACA |
| qpcr-sec15-r | ACACCGTTGGCATTTGGAAA |
| qpcr-ypt32-f | CAGTACCAACCGATGAAGCG |
| qpcr-ypt32-r | CCGACCCGCTTAAGTCTACT |
| qpcr-sec16-f | ACGTTCAGGCCCCATCAATA |
| qpcr-sec16-r | GGGAGAGGATGGTGATGGAG |