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Mammalian Brd4 protein rescues E2-mediated plasmid maintenance. Red/white sectoring assay of YMB1670 transformants containing p416CYC1-E2, indicated reporter plasmids (see Fig. 1B), and empty vectors (p425/PGK and pADNS) or expression vectors for mammalian candidate proteins p425PGK-hEBP2 (p40), pADNS-Brd2, or pADNS-Brd4. Transformants were streaked onto media nonselective for the reporter plasmids.
