Fig. 4.

Brd4 maintains plasmids containing E2RE1 or LCR elements. (A) Red/white sectoring assay of YMB1670 transformants containing p416CYC1E2, pADNS-Brd4, and individual reporter plasmids as indicated (see Fig. 1B). Transformants were streaked onto media selective and nonselective for the reporter plasmids. (B) Individual colonies of transformants shown in A on nonselective medium. (C) Plasmid-loss assay of YMB1670 transformants shown in A. After eight generations of growth in nonselective media, 10-fold serial dilutions were plated on media selective and nonselective for the reporter plasmids.