Fig. 5.

Requirements for E2-mediated plasmid maintenance. Red/white sectoring assay of YMB1670 transformants with pADNS-Brd4, individual reporter plasmids (see Fig. 1B), and p416CYC1 plasmids expressing WT or mutated E2 proteins. Transformants were streaked on media nonselective for the reporter plasmids. The phenotypes of the E2 proteins are indicated as transactivation (ta), replication (rep), DNA binding (db), and chromosome binding (cb) and are listed in more detail in Table 2.