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. 2017 Jul 3;3:17033. doi: 10.1038/cddiscovery.2017.33

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Distribution of cell cycle phase in HW1 and RN1 cell lines treated with palbociclib. (a, b) DNA histograms generated by flow cytometry showing the distribution of cells in various stages of the cell cycle for cell lines HW1 and RN1. Cells were treated with either DMSO vehicle (control) or 4 μM palbociclib (treated) for 48 h. Histograms are representative of n=3 experiments. (c, d) Graphical representation of DNA histograms for HW1 and RN1 cell lines showing the percentage of cells in the G0/G1, S-Phase and G2/M phases of the cell cycle (n=3 experiments).