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Immunoblot and lectin blot analyses of the FLAG-β1-subunit expressed in Sf-9 cells. The blot containing purified FLAG-β1-subunit proteins was incubated with anti-FLAG antibody (lane A), anti-rat β1-subunit antibody (lane B), or Con A (lane C). The purified FLAG-β1-subunit proteins were subjected to GlcNAc-agarose column chromatography, and blots containing proteins in the pass-through (lane D) and bound (lane E) fractions were incubated with anti-rat β1-subunit antibody.
