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. 2005 Feb 10;102(8):2796–2801. doi: 10.1073/pnas.0409344102

Fig. 3.

Fig. 3.

Effect of K+ concentration in the buffer on the binding of the 38- and 40-kDa FLAG-β1-subunit proteins to GlcNAc-agarose. Purified FLAG-β1-subunit proteins were applied to a GlcNAc-agarose column in the presence of increasing concentrations of KCl. Relative bindings of the FLAG-β1-subunit to the column were determined by anti-rat β1-subunit antibody reactivity toward the 38- and 40-kDa proteins in the bound fractions. Three independent experiments were conducted, and the mean values are shown with standard errors.