Table 1. Relative binding specificities of the FLAG-β1-subunit toward carbohydrates.

Glycoprotein-Sepharose or carbohydrate-agarose	Relative binding
AsAg-transferrin-Sepharose	+++
AsAgAgn-transferrin-Sepharose	-
As-transferrin-Sepharose	-
Transferrin-Sepharose	-
GlcNAc-agarose	+++
GalNAc-agarose	+
Mannose-agarose	-
Lactose-agarose	-

The relative bindings are shown by scoring band intensities of the proteins recovered from the GlcNAc-agarose column as +++ by nih image analysis after detection with the antibody.