A) Coherent anti-Stokes Raman scattering (CARS) multiphoton imaging of cellular lipid droplets in peritoneal macrophages treated with miR-33 and control mimics and treated with acLDL for 24h. Quantification of lipid droplet volume by voxel analysis is shown at right. Scale bar = 10 μm. B) Cellular efflux of 3H-cholesterol to apolipoprotein A-I (apoA-I, 50μg/mL) in peritoneal macrophages treated with control or miR-33 mimics, in the presence or absence of the neutral lipolysis inhibtor paraoxon (Neutral) or the lysosomal inhibitor chloroquine (Lysosomal). C) Cellular efflux of 3H-cholesterol to apoA-I as in (B), in peritoneal macrophages treated with control or anti-miR-33 inhibitors.. *P < 0.05, **P < 0.01 compared to control treatment. NS, not significant.