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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2017 Apr 20;37(6):1058–1067. doi: 10.1161/ATVBAHA.116.308916

Figure 3. miR-33 regulation of cholesterol efflux requires autophagy.

Figure 3

A, B) Cellular efflux of 3H-cholesterol to apoA-I in wild type and Atg5−/− macrophages treated with miR-33 and control (A) mimics or inhibitors (B). C) Immunofluorescence imaging of Bodipy-stained lipid droplets in Ctrl anti-miR or anti-miR-33 treated wild type and Atg5−/− macrophages loaded with acetylated LDL. Cells were imaged prior to (control) or after incubation with apoA-I for 4h to promote cholesterol efflux. Scale bar, 25μm. D) Quantification of neutral lipid content of cells shown in (C). E) qRT-PCR of Abca1 mRNA in cells shown in (C). *P<0.05, **P<0.005. NS, not significant.