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. 2005 Feb 11;102(8):2802–2807. doi: 10.1073/pnas.0409373102

Fig. 3.

Fig. 3.

HIPK2 involvement in UV-mediated Ser-422 phosphorylation. (a) siRNA to HIPK2 (HIPK2 siRNA) attenuates Ser-422 phosphorylation of endogenous CtBP in H1299 cells in response to UV irradiation. The empty vector, pH1, served as control. Cells were stained with the anti-phospho-Ser-422 Ab. (b) UV-triggered Ser-422 phosphorylation blocked by HIPK2 siRNA. HIPK2 expression in COS-7 cells was perturbed by siRNA (+). The empty vector, pH1, served as control (–). CtBP expressed in COS-7 cells with (+) or without (–) UV irradiation was monitored by Western blotting using the phospho-specific (pCtBP) or CtBP Abs. (c) Basal phosphorylation of CtBP at Ser-422. CtBP from HeLa nuclear extract (N) was compared with recombinant CtBP (S). Bacterially purified CtBP served as titration standard for the nonphosphorylated protein. Samples were subjected to Western blotting with the phospho-specific (pCtBP) or CtBP Abs.