Fig. 2.

Induction of bioactive IL-12, IL-18, and proinflammatory cytokines in MDCs treated with Lactobacillus cells. MDCs were treated with Lactobacillus species, E. coli LPS, or no supplement for 3 d at 37°C. (A) Supernatants of MDCs treated with Lactobacillus species, E. coli LPS, or no supplement were harvested after 72 h and analyzed for IL-10 and IL-12 by ELISA. Experiments were performed at least three times with similar results. (B) MDCs were treated with Lactobacillus species, E. coli LPS, L. reuteri and E. coli LPS, or no supplement for 48 h. MDCs were harvested and treated with Golgi inhibitor for 4 h. Cells were stained with anti-IL-10 allophycocyanin or anti-IL-12 FITC for 1 h on ice. Cells were washed and analyzed by flow cytometry. (C) DCs were treated with LPS alone or in combination with Lactobacillus species for 72 h. Cytokines were then analyzed by ELISA. (D) Supernatants of MDCs treated with Lactobacillus species, E. coli LPS, or no supplement were harvested after 72 h and analyzed for IL-18 by ELISA. Experiments were performed at least three times with similar results. (E–H) MDCs supernatants were harvested and analyzed for proinflammatory cytokines by ELISA. Results are representative of three independent experiments.