FIG 1.

Pigmentation of wild-type and putative glycosyltransferase mutant strains on blood agar. (A to D) Close-up images of individual colonies of W83 (wild type) and the putative glycosyltransferase deletion mutant (Δ-PG0264), the putative glycosyltransferase transposon mutant (Tn-PG0264), and the complemented deletion mutant (Δ-PG0264 +PGN_0361) in the W83 strain background are shown. (E) Heme bound to W83, ΔPG_0264, and ΔPG_0264 complemented (Δ0264 Comp) strains was quantified from colonies grown on blood agar plates for 6 days. Cells were resuspended in PBS and adjusted to equivalent optical densities. Heme was quantified from 50 μl of these cell suspensions. Shown are the means ± standard errors from three independent experiments, each performed in triplicate. Statistical significance was determined by two-tailed t test (*, P < 0.05; **, P < 0.01).