FIG 2.

Growth curves of wild-type and pigmentation-defective mutant strains. Forty-eight-hour cultures of each strain were diluted into fresh BHIHKS_bcS_tgC medium to an OD₆₀₀ of 0.02. Growth was monitored under anaerobic conditions for 48 h. Strains include W83 (wild type), a lysine gingipain transposon mutant (TnKgp), an O-antigen ligase transposon mutant (TnWaaL), a PG_0264 glycosyltransferase transposon mutant (Tn0264), a PG_0264 glycosyltransferase deletion mutant (Δ0264), a complemented PG_0264 glycosyltransferase deletion mutant (Δ0264 Comp), and a complemented PG_0264 transposon mutant (Tn0264 Comp). The growth curves represent the averages from three independent experiments. Error bars represent the standard errors. Statistical significance was determined by two-way analysis of variance (ANOVA) and corrected for multiple comparisons (P < 0.05).