TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Descriptiona | Antibiotic resistanceb | Source |
|---|---|---|---|
| S. pneumoniae strains | |||
| D39 | NCTC7466, serotype 2, encapsulated, TH4533 | NCTC | |
| D39s | D39 derivative, rpsL1, TH4527 | Sm | 73 |
| ST556 | S. pneumoniae type 19F | 45 | |
| TH4737 | D39s derivative, ΔptvR-C::JC (replacement of entire ptvR-C coding region with JC) | Kan | This study |
| TH4631 | D39s derivative, ΔptvR::JC (replacement of ptvR coding region with JC) | Kan | This study |
| TH5139 | D39s derivative, JC insertion in front of ptvR start codon | Kan | This study |
| TH6854 | TH5031 derivative, ΔptvR ΔptvC::JC (replacement of ptvC with JC in TH5031) | Kan | This study |
| TH6855 | TH5031 derivative, ΔptvR ΔptvB::JC (replacement of ptvB with JC in TH5031) | Kan | This study |
| TH6910 | TH5031 derivative, ΔptvR ΔptvA::JC (replacement of ptvA with JC in TH5031) | Kan | This study |
| TH4741 | TH4737 derivative, ΔptvR-C (unmarked deletion of the entire ptvR-C coding region) | Sm | This study |
| TH5031 | TH4631 derivative, ΔptvR (unmarked deletion of ptvR) | Sm | This study |
| TH5204 | TH5139 derivative, unmarked insertion of P-spxB immediately before first nucleotide of ptvR start codon | Sm | This study |
| TH5205 | Same as TH5204, clonal sibling | Sm | This study |
| TH6916 | TH5031 derivative, ΔptvR ΔptvA (unmarked deletions of ptvR and ptvA) | Sm | This study |
| TH6868 | TH5031 derivative, ΔptvR ΔptvB (unmarked deletions of ptvR and ptvB) | Sm | This study |
| TH6856 | TH5031 derivative, ΔptvR ΔptvC (unmarked deletions of ptvR and ptvC) | Sm | This study |
| TH6908 | D39s derivative D39s::pTH6862 containing episomal ptvR in pTH6862 | Cm, Sm | This study |
| TH6881 | TH5031 derivative TH5031::pTH6862 containing episomal ptvR in pTH6862 | Cm, Sm | This study |
| TH6876 | TH5204 derivative TH5204::pTH6862 containing episomal ptvR in pTH6862 | Cm, Sm | This study |
| TH6906 | D39s derivative D39s::pIB166 containing empty plasmid pIB166 | Cm, Sm | This study |
| TH6879 | TH5031 derivative TH5031::pIB166 containing empty plasmid pIB166 | Cm, Sm | This study |
| TH6875 | TH5204 derivative TH5204::pIB166 containing empty plasmid pIB166 | Cm, Sm | This study |
| TH9753 | TH4527 derivative, ptv::luc | Kan | This study |
| TH9754 | TH5031 derivative, ptv::luc | Kan | This study |
| TH9755 | TH4527 derivative, ptv::luc bgaA::PZn2+-ptvR | Kan, Tet | This study |
| TH9756 | TH5031 derivative, ptv::luc bgaA::PZn2+-ptvR | Kan, Tet | This study |
| MK134 | D39 PssbB-ssbB_luc Kanr | Kan | 70 |
| E. coli TH5272 | Rosetta containing pET22b::ptvR | Amp | This study |
| Plasmids | |||
| pIB166 | E. coli-S. pneumoniae shuttle vector | Cm | 69 |
| pTH6862 | pIB166 derivative expressing C-terminally His-tagged PtvR driven by spxB promoter, pIB166::ptvR | Cm, Sm | This study |
| pTH2700 | pGEX-2T derivative with a modified multiple-cloning site | Amp | 77 |
| pTH2604 | pTH2700 derivative pTH2700::ptvB expressing N-terminally GST-tagged PtvB | Amp | This study |
| pTH2760 | pTH2700 derivative pTH2700::spxB expressing N-terminally GST-tagged SpxB | Amp | This study |
a
JC, Janus cassette.
b
Antibiotic abbreviations: Amp, ampicillin; Cm, chloramphenicol; Kan, kanamycin; Sm, streptomycin; Tet, tetracycline.