Figure 4.

Upregulation of p73, PUMA and Bak expression by curcumol in TNBC MDA-MB-231 cells. (A) Immunoblotting demonstrated increased protein levels of p73, PUMA, Bak and cleaved PARP in MDA-MB-231 cells following treatment with 25 or 100 µg/ml curcumol for 24 h. Actin was the loading control, and ADM-treated MCF-7 cells served as the antibody controls. Cleavage of PARP is indicated by the arrow. (B) Quantitative data for the relative density of the proteins of interest. Each bar represents the mean ± standard deviation of three independent experiments (*P<0.05, **P<0.01 vs. the untreated control). PUMA, p53 upregulated modulator of apoptosis; Bak, Bcl-2 antagonistic killer; PARP, poly (ADP-ribose) polymerase; ADM, Adriamycin; MCF, macrophage chemotactic factor; TNBC, triple-negative breast cancer.