Table 1. Properties of copy-up RepA mutants.
| miniP1ΔincA copy number*
|
PrepA repression,† % (RepA in trans)
|
[RepA] in cis (Western)
|
|||||
|---|---|---|---|---|---|---|---|
| RepA | WT/miniF | ΔdnaJ/miniF | WT/miniF + incA | WT | ΔdnaJ | WT | ΔdnaJ |
| WT | 1 | 0.7 | 0 | 97 | 76 | 1 | 3.9 |
| D55G | 1.9 | 3.0 | 2.2 | 98 | 97 | ||
| A56T | 2.1 | 1.8 | 1.5 | 96 | 92 | ||
| A117G | 1.3 | 1.3 | 0.7 | 99 | 92 | ||
| F120L | 1.2 | 1.1 | 0.3 | 54 | 44 | 2.3 | 11 |
| K123G | 1.3 | 1.0 | 1.2 | 30 | 19 | 4.3 | 23 |
| K143E | 1.5 | 1.6 | 1.1 | 49 | 36 | 3.1 | 12 |
| D152V | 1.2 | 1.3 | 0.4 | 94 | 82 | ||
| F167S | 1.6 | 3.0 | 1.3 | 97 | 83 | ||
| D172E | 1.2 | 1.3 | 0.4 | 85 | 69 | ||
| D180G | 1.5 | 1.2 | 0.8 | 98 | 83 | ||
| K181E | 1.3 | 1.1 | 0.4 | 92 | 80 | ||
The three mutants shown in bold were not only chaperone-dependent but also DNA-binding defective. WT also is shown in bold.
*
Copy numbers are relative to the WT plasmid pSP102 and are mean values from three cultures started from independent colonies. The average one SD was ≈20% of the mean.
†
The repA promoter was fused to lacZ and was present in one copy in the chromosome. RepA was supplied in trans from constitutive promoter roughly at physiological concentration. The average one SD was ≈12% of the mean.