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. 2017 May 5;14(1):35–40. doi: 10.3892/ol.2017.6127

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Heterogeneous 4-HNE expression level in ESCC. (A) IHC analysis was performed on formalin-fixed paraffin-embedded tissues, and the expression levels of 4-HNE were investigated in the cytoplasm. Based on the staining, tumors in the cohort were categorized into (a) 4-HNE-positive or (b) 4-HNE-negative in ESCC tissues; the normal epithelium was categorized into (c) 4-HNE-positive or (d) 4-HNE-negative; carcinoma in situ was categorized into (e) 4-HNE-positive or (f) 4-HNE-negative. (B) IHC score of 4-HNE expression in ESCC tissues in situ and benign tissues. IHC staining scale bar, 50 µm. *P<0.05. 4-HNE, 4-hydroxynonenal; ESCC, esophageal squamous cell carcinoma; IHC, immunohistochemistry.