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. 2017 May 5;14(1):35–40. doi: 10.3892/ol.2017.6127

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Expression level of 4-HNE in ESCC tissues with a variety of inflammation severities. (A) The expression level of 4-HNE in (a) normal esophageal epithelial is negative and (b) the expression of carcinoma in situ tissues with inflammation is positive for 4-HNE. (c) In the same tissue, the expression level of 4-HNE is higher in epithelial tissues with inflammation compared with that of normal epithelial tissues. (B) Expression level of 4-HNE in ESCC tissues with a variety of inflammation severity. (a) Tissues with severe inflammation demonstrated a high expression level of 4-HNE, and (b) low 4-HNE expression level and (c) negative 4-HNE staining was identified in tissues with no inflammation. (C) The IHC score of 4-HNE was determined in ESCC tissue subgroups with a variety of inflammation severity. The IHC score of 4-HNE in severe inflammation is significantly higher compared with normal tissues. IHC staining scale bar, 50 µm. *P<0.05.