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WA inhibits the growth of AGS cells by inducing apoptosis. The apoptotic status of AGS cells after (A) 6 h and (B) 18 h was evaluated using Annexin V-fluorescein isothiocyanate/propidium iodide double-staining method in flow cytometry. (C) Cellular protein was harvested at 6 h and the amounts of cleaved caspase-3, −7 and −9, PARP, Bcl-2, and β-actin were determined by western blotting. WA, withaferin A; AGS, gastric adenocarcinoma; PARP, poly(ADP-ribose) polymerase.
