Figure 3.

WA induces G2/M cell cycle arrest by decreasing the expression of the cell cycle regulatory protein cyclin B1 in AGS cells. (A) The effect of WA on cell cycle distribution in AGS cells was evaluated using a propidium iodide staining assay. Results indicate the proportion of cells in each phase following treatment with WA (0, 1, 2.5 or 5 µM) for 12 h. (B) The expression of cyclin B1 following 2.5 µM WA treatment of AGS cells was detected using western blot analysis at various time points (0, 3, 6, 12 and 24 h). Data are representative of three independent experiments. WA, withaferin A; AGS, gastric adenocarcinoma.