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Internalization of EGFR at different doses of EGF. (A) Immunoelectron microscopy of EGFRwt in HeLa cells in coated pits (CP), coated vesicles (CV), caveolae (CAV), or caveosome-like structures (CVS). (Bar, 111 nm.) (B) Lysates (1 mg) from HeLa cells were immunoprecipitated (IP) or directly loaded (50 μg) and immunoblotted (IB) with the indicated antibodies.
