Table 2.
SPECIFICITY | CLONE | FLUOROCHROME | PURPOSE |
---|---|---|---|
Live/dead | N/A | Near infrared | Viability |
CD45 | D058-1283 | BV786a | Nonhuman primate specific hematopoietic cell lineage |
CD3 | SP34-2 | PE-CF594 | Exclusion |
CD20 | 2H7 | PE-CF594 | |
CD8 | RPA-T8 | BV711 | NK subsets |
CD16 | 3G8 | Pacific Blue | |
CD56 | B159 | PerCP-Cy5.5 | |
NKG2A/C | Z199 | PE-Cy7 | |
KIR3DL01 | NKVFS1 | PE | |
KIR3DL05 | GY9 tetramer | APC | |
IFN-γ | 4S.B3 | FITC | NK cell function |
TNF-α | MAb11 | Alexa700 | |
CD107a | H4A3 | BV605 | |
Granzyme B | GB11 | BV510 |
BV is brilliant violet.