Table II.

Primer and probe sequences used in the MethyLight assay for APC, CYP26A1, HOXD3, HOXD8, RASSF1, TBX15, TGF-β genes and ALU control reaction.

Genea,b	Sequence
ALU	Forward: 5′-GGTTAGGTATAGTGGTTTATATTTGTAATTTTAGTA-3′
	Reverse: 5′-ATTAACTAAACTAATCTTAAACTCCTAACCTCA-3′
	Probe: 5′FAM-CCTACCTTAACCTCCC-3′BHQ1
APC	Forward: 5′-GAACCAAAACGCTCCCCAT-3′
ENSG000001349821	Reverse: 5′-TTATATGTCGGTTACGTGCGTTTATAT-3′
Chr5: 112737781-1127378562	Probe: 5′FAM-CCCGTCGAAAACCCGCCGATTA-3′BHQ1
CYP26A1	Forward: 5′-TTGTAGAGATTCGACGTACGCGG-3′
ENSG00000095596	Reverse: 5′-AAAACCTTCCGTCAAACATCCTCTACG-3′
Chr10: 93069103-93069230	Probe: 5′FAM-ACGCCCACGACGTACCCGCTTCCTTAC-3′BHQ1
HOXD3	Forward: 5′-TTAAAGGTTTATGGTTGCGC-3′
ENSG00000128652	Reverse: 5′-TTACGAACACTAAACTACACCCG-3′
Chr2: 176163186-176163274	Probe: 5′FAM-ACAAAACGTTCCCGACGCTTCTAAAA-3′BHQ1
HOXD8	Forward: 5′-TAGTCGGTTTTGGTTCGTTGC-3′
ENSG00000175879	Reverse: 5′-CGTTCTAAAACGAAAAAAAAAACTCGCG-3′
Chr2: 176128934-176129040	Probe: 5′FAM-TCCTCGAACAAAACGCGACTCCCGAATCTC-3′BHQ1
RASSF1	Forward: 5′-ATTGAGTTGCGGGAGTTGGT-3′
ENSG00000068028	Reverse: 5′-ACACGCTCCAACCGAATACG-3′
Chr3: 50340720-503407843	Probe: 5′FAM-CCCTTCCCAACGCGCCCA-3′BHQ1c
TBX15	Forward: 5′-GCGGTTTTGTAAGTATATTGTTGCG-3′
ENSG00000092607	Reverse: 5′-ACTCCGAATAAAACAAAAACTAAAATCCG-3′
Chr1: 118984438-118984535	Probe: 5′FAM - CAAATAACGCCGCCGAACGCCT-3′BHQ1
TGF-β	Forward: 5′-TTTTAGGAGAAGGCGAGTCG-3′
ENSG00000092969	Reverse: 5′-CTCCTTAACGTAATACTCTTCGTCG-3′
Chr1: 218346933-218347007	Probe: 5′FAM-TCTCGCGCTCGCAAACGACC-3′

^aEnsembl gene accession number.

^bHuman genome assembly GRCh38/hg38.

^cThe RASSF1A TaqMan hydrolysis probe sequence contains the SNP rs4688725. APC, adenomatous polyposis coli; CYP26A1, cytochrome P450 family 26 subfamily A member 1; HOX, homeobox; RASSF1, Ras association domain family member 1; TBX15, T-box 15; TGFβ2, transforming growth factor β2; ALU, Arthrobacter luteus element.