Table II.
Thermocycling program for quantitative polymerase chain reaction.
| Step | Temperature, °C | Duration |
|---|---|---|
| 1 | 95 | 10 min |
| 2 | 95 | 10 sec |
| 3 | 55 | 15 sec |
| 4 | 72 | 30 sec |
| Steps 2–4 | – | 40 cycles |
| Melt curve program | – | 60 min |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.