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. 2005 Feb 11;102(8):2874–2879. doi: 10.1073/pnas.0500187102

Fig. 1.

Fig. 1.

NF-κB activation and TRAF recruitment by CD40 and CD40 mutants. (A) Schematic presentation of the TRAF interaction sites in the CD40-signaling domain. Shown are the interactions sites of TRAF6 and TRAF2/3/5 as well as the mutations relevant in this study. (B) CD40-mediated activation of NF-κB ceases only when the TRAF6, the TRAF2/3/5, and two further QE motifs in the CD40 C terminus are mutated or deleted (correlation with TRAF recruitment). All assays were done as described in Materials and Methods. Induction of NF-κB activity was determined after 3 h of stimulation with CD40L+ cells. The results were normalized on the basis of receptor expression. TRAF recruitment is expressed as the percentage of the CD40wt control (arbitrarily assumed to be 100%).