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. Author manuscript; available in PMC: 2018 Jul 15.
Published in final edited form as: Biochem Pharmacol. 2017 Apr 9;136:86–98. doi: 10.1016/j.bcp.2017.03.025

Figure 10. LDHA silencing sensitizes HCT116 cells to oxidative stress and increases the proportion of apoptotic cells after GYY4137 treatment.

Figure 10

HCT116 cells (either with scrambled siRNA treatment or with siRNA-mediated LDHA silencing) were treated with vehicle or GOx (0.01–0.1 U/mL) for 1 h. Next, the cells were washed and the medium was replaced with vehicle or GYY4137 (0.3 mM) and incubated for another 24 h. (A) MTT reduction in HCT116 transiently transfected with control or siRNA specific LDHA treated under various conditions. (B) Necrotic cell death determined by LDH release in control or siLDHA transfected HCT116 under various treatment conditions. Data represent mean ± SEM and expressed as a percentage of the corresponding siRNA CTL group. (C) Graph represents changes in subpopulations of apoptotic cells. Solid bars reflect siRNA groups while stripped bars denote siLDHA groups. Total cell number in each group was set to 100%. (D) Apoptotic rate was calculated by dividing the sum of stained cells to live cells. n = 5–8, for each group (A); n = 6, for each group (B); n = 3, for each group (C,D). *P < 0.05 vs. siRNA CTL, #P < 0.05 vs. siLDHA CTL; ‡P < 0.05 vs. siLDHA GYY4137 group; †P < 0.05 (based on two-way ANOVA corrected with Tukey’s post-hoc test).