Skip to main content
. Author manuscript; available in PMC: 2018 Jul 15.
Published in final edited form as: Biochem Pharmacol. 2017 Apr 9;136:86–98. doi: 10.1016/j.bcp.2017.03.025

Figure 4. H2S stimulates mitochondrial respiration in HCT116 cells; this response requires functional LDHA.

Figure 4

HCT116 cells (either with scrambled siRNA treatment or with siRNA-mediated LDHA silencing) were treated with GYY4137 (0.1, 0.3 or 1 mM) for 24 h, followed by Extracellular Flux Analysis. OCR in HCT116 cells transfected with either nontargeting (A) or siLDHA (B) is shown. C–H: Comparison of cellular bioenergetics parameters. Data represent mean ± SEM and expressed as a percentage of the corresponding siRNA CTL group. n = 3–6 for each group. *P < 0.05 vs. siRNA CTL; #P < 0.05 vs. siLDHA CTL (based on two-way ANOVA (A, B), or one-way ANOVA (C–H) corrected with Tukey’s post-hoc test).