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. Author manuscript; available in PMC: 2018 Jul 15.
Published in final edited form as: Biochem Pharmacol. 2017 Apr 9;136:86–98. doi: 10.1016/j.bcp.2017.03.025

Figure 7. H2S stimulates the catalytic activity of wild-type LDHA catalytic activity, but not the activity of C163A LDHA.

Figure 7

(A) Protein sequences of WT LDHA and C163A LDHA. Protein sequences of WT LDHA and C163A LDHA. A His tag was added to the end of each protein to aid in the protein isolation and purification steps (letters shown in red). The Cys residue substituted to Ala in C163A LDHA is shown bold and highlighted. (B) WT LDHA or C163A LDHA (1 μg) were pre-incubated with 10–300 μM NaHS for 30 min at 37°C. LDHA enzymatic analysis was carried out as described in the Materials and Methods. Data represent mean ± SEM and expressed as a percentage of the corresponding untreated WT LDHA group. n = 4–6, for each group; *P < 0.05 vs. WT LDHA CTL (based on one-way ANOVA corrected with Tukey’s post-hoc test).