Table 3. Genetic control of seed mass and yield by AP2.
| Parental genotypes
|
F1 seed genotype
|
Average seed mass
|
|||||
|---|---|---|---|---|---|---|---|
| ♀ | ♂ | SP | EN | EM | F1 | F2 | Average F2 seed yield |
| -/- | -/- | -/- | -/-/- | -/- | 4.75 (0.07) | ND | ND |
| -/- | +/+ | -/- | -/-/+ | -/+ | 3.65 (0.14) | 2.84 (0.21) | 2.70 (0.35) |
| +/+ | -/- | +/+ | +/+/- | +/- | 2.60 (0.04) | ND | ND |
| +/+ | +/+ | +/+ | +/+/+ | +/+ | 2.30 (0.10) | 2.46 (0.15) | 2.00 (0.22) |
| +/- | -/- | +/- | +/+/- | +/- | 3.13 (0.12) | ND | ND |
| +/- | -/-/- | -/- | |||||
| +/+ | AS/AS | +/+ | +/+/AS | +/AS | 3.50 (0.26) | ND | ND |
The + and - designations indicate the presence of the wild-type AP2 and the recessive mutant ap2-10 alleles, respectively. AS indicates the AP2 antisense transgene in pollen donor PW15-3 (C24) that confers an ap2 seed size phenotype as described in Table 2 Standard deviation values are shown in parentheses. Seed mass is given in units of mg per 100 seeds. Seed yield is the average weight (g) of the total number of F2 seeds produced per plant for WT (n = 7) and ap2-10(+/-) (n = 9) F1 plants. SP, EN, and EM refer to the maternal sporophyte, endosperm, and embryo genotypes, respectively. ND, not determined.