Table 1. Compounds that have been used for in vitro CYP450 profiling studies in micropatterned cocultures (MPCCs).

All substrates can be reconstituted in dimethyl sulfoxide (DMSO) and incubated in MPCCs at the indicated concentrations for 1 hour. The supernatants are then collected and stored at -80°C prior to LC-MS/MS analysis or luminescence analysis (for the CYP2C9-Glo and CYP3A4-Glo substrates from Promega).

Enzyme	Concentration (μM)	Substrate	Metabolites
1A2	100	Phenacetin	Acetaminophen
2A6	50	Coumarin	7-OH-coumarin
2B6	500	Bupropion	OH-bupropion
2C8	30	Paclitaxel	6a-OH-paclitaxel
2C9	50	Tolbutamide	4-OH-tolbutamide
2C9	100	Diclofenac	4-OH-diclofenac
2C9	100	CYP2C9-Glo	Luciferin
2C19	100	S-mephenytoin	4-OH-S-mephenytoin
2D6	16	Dextromethorphan	Dextrorphan
2E1	300	Chlorzoxazone	6-OH-chlorzoxazone
3A4	200	Testosterone	6b-OH-testosterone
3A4	3	CYP3A4-Glo (luciferin-IPA)	Luciferin
Phase II Metabolism	50	7-OH-coumarin	7-OH-coumarin glucuronide and 7-OH-coumarin sulfate