Transgenic chloroplasts of Marchantia polymorpha expressing the cyan fluorescent protein mTurquoise2 under control of the tobacco psbA promoter. Mature thallus of Marchantia was mounted in water under a coverslip, and examined under a 63×, NA 1.2 objective using a Leica SP5 confocal laser-scanning microscope. Cells surrounding an air pore were imaged using 458-nm laser excitation. Emission wavelengths were collected for chloroplast autofluorescence (610–700 nm, left) and cyan fluorescent protein (CFP) fluorescence (466–495 nm, right), with merged images in red and green channels, respectively, shown center. Scale bar, 20 µm.