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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cancer Res. 2017 Apr 5;77(11):2834–2843. doi: 10.1158/0008-5472.CAN-16-2238

Figure 2. IRF8 is silenced but iNOS is upregulated in MDSCs of tumor-bearing mice.

Figure 2

A. CD11b+Gr1+ cells were purified from spleens of tumor-free (n=4) and 4T1 tumor-bearing (n=5) BALB/c mice and analyzed by qPCR for IRF8 mRNA level. B. CD11b+Gr1+ cells were purified from spleens of tumor-free (n=3) and 4T1 tumor-bearing (n=3) BALB/c mice and analyzed by qPCR for iNOS mRNA level. C. Tumors were excised from AT3 tumor-bearing WT (n=3) and IRF8 KO (n=3) C57BL/6 mice and a single cell suspension was prepared. Tumor mixtures were then sorted for infiltrating CD11b+Gr1− and CD11b+Gr1+ cells. Top panel shows gating of sorted cells. Bottom panel shows analysis of the sorted cells by qPCR for iNOS mRNA level.