Figure 4. SETD1B and H3K4me3 levels are elevated in tumor-induced MDSCs.

A. 4T1 tumor cells were injected s.c. into the mammary glands of BALB/c mice. Spleens were collected from tumor-free (TF, n=3) and 4T1 tumor-bearing (TB, n=3) mice. CD11b⁺Gr1⁺ cells were purified from spleen cells and analyzed for H3K4me3 level by Western blotting. Histone 3 (H3) and β-actin proteins were used as normalization controls. B. CD11b⁺Gr1⁺ cells were purified from the spleens of TF (n=4) and TB (n=5) mice and analyzed by RT-PCR with gene-specific primers as indicated. β-actin was used as a normalization control. C. CD11b⁺Gr1⁺ cells were purified from spleens of TF (n=3) and TB (n=3) mice and analyzed by qPCR for SETD1B expression level.